Journal: mAbs

Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance

doi: 10.1080/19420862.2026.2674236

Figure Lengend Snippet: T-cell activation and antitumor activity of surrogate 1 + 1 and 2 + 1 H10 TCEs. a) Binding of H10-sTCE1 + 1 and H10-sTCE2 + 1 to isolated mouse splenocytes. b) Activation of CD4+ and CD8+ splenocytes by TCEs in co-culture with CT-26 EGFRwt cells. c) Tumor growth inhibition mediated by different doses of H10-sTCE1 + 1 and H10-sTCE2 + 1 in a CT-26 EGFRwt syngeneic model in mice. d) Comparison of tumor volumes at day 17.

Article Snippet: For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461).

Techniques: Activation Assay, Activity Assay, Binding Assay, Isolation, Co-Culture Assay, Inhibition, Comparison